DIG-High Prime DNA標記及檢測啟動裝I試劑

DIG-High Prime DNA標記及檢測啟動裝I試劑

產品描述

General description

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids.

DIG系統被建立為一種敏感且具有成本效益的替代放射性的核酸標記和檢測系統。有許多可用的出版物證明了DIG系統的多功能性，因此使用無線電標記不再是標記雜交DNA的選擇。

Application

DIG-High Prime DNA Labeling and Detection Starter Kit I has been used in a variety of hybridization techniques:

• in Southern blots[1][2]

• in northern blots

• in dot blots[3]

• in colony and plaque hybridizations

• for all types of filter hybridization

• for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley and wheat

DIG-labeled probes are generated at high yield within one hour or after overnight incubation.

Features and Benefits

1個試劑盒包含7種組分。

Quality

已通過斑點雜交試驗測試功能。

Other Notes

僅用于生命科學研究。不可用于診斷。

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